Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt
نویسندگان
چکیده
This study aimed to evaluate the most predominant strain of avian pathogenic Escherichia coli (APEC) that causes colibacillosis with respiratory manifestation in broiler chickens. Isolation and serotyping identification for isolates E. were performed. Prevalence APEC differentiated by detection 8 virulence-associated genes “astA, iss, irp2, papC iucD, tsh, vat, cva/cvi” using multiplex PCR technique important was also investigated. Antibacterial sensitivity test antibiotics disc oregano at different concentrations (20%, 15%, 10%, 5%), estimated coli. A total 50 farms located Gharbia, Giza, Menoufia, Bahaira, Dakahliah Governorates examined. Specimens from lung, liver, spleen, kidney diseased birds used bacteriological examination. Results revealed O27 serotype (11 out 35 isolates), followed serotypes O146 (4/35), O Untypable (1/35) each O8, O20, O44, O78, O114, O125, O142, O152, O153, O158, O164, O166. One isolate 11 has 6 virulence-related papC, cva/cvi”. Difloxacin a concentration 20% have highest percentage (100%) against strains. In conclusion, infecting could be treatment O27.
منابع مشابه
Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail
Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is an economic threat to the poultry industry throughout the world. Some of the virulence genes may enhance the ability of E. coli isolates to grow in the tissues of broilers. The APEC strains are assigned to a few distinct phylogenetic groups. The purpose of the present study was to detect the virulence genes a...
متن کاملIn vitro assessment of pathogenicity and virulence encoding gene profiles of avian pathogenic Escherichia coli strains associated with colibacillosis in chickens
Background: Avian pathogenic Escherichia coli (APEC) strains have been associated with various disease conditions in avian species due to virulence attributes associated with the organism. Aims: This study was carried out to determine the in vitro pathogenic characteristics and virulence encoding genes found in E. coli strains associated with colibacillosis in chickens. Methods: Fifty-two stock...
متن کاملA study on the presence of some potential virulence genes and quinolone resistance in avian pathogenic Escherichia coli isolated from chickens in Northeast of Iran
Background: Avian pathogenic Escherichia coli (APEC), which is the causative agent of colibacillosis, harbors several putative virulence genes. An important trait of APEC for both poultry and public health is antibiotic resistance. Objectives: In the present study, some potential virulence genes of APECs isolated from Northeast of Iran and their resistance to the quinolones antibiotics were stu...
متن کاملIsolation, Identification and in vitro Susceptibility of Avian Escherichia coli to Selected Fluoroquinolones
متن کامل
Phenotypic and genotypic characterization of antimicrobial susceptibility of avian pathogenic Escherichia coli isolated from broiler chickens
Aim Avian pathogenic Escherichia coli (APEC) is pathogenic strains of E. coli that are responsible for one of the most common bacterial diseases affecting poultry worldwide. This study was designed to determine the occurrence, antibiotic resistance profile, and antibiotic resistance genes of E. coli isolated from diseased and freshly dead broilers. Materials and Methods In that context, a tot...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Egyptian Journal of Veterinary Science
سال: 2022
ISSN: ['1110-0222', '2357-0903', '2357-089X']
DOI: https://doi.org/10.21608/ejvs.2021.93246.1274